The Nephrocystin-3 Polyclonal Antibody (CAB8580) is a valuable tool for researchers studying Nephrocystin-3, a protein implicated in the development of kidney and liver diseases. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to Nephrocystin-3, researchers can detect and analyze the protein in various cell types, making it an essential component for studies in nephrology and hepatology research.
Nephrocystin-3 plays a crucial role in maintaining the structure and function of the kidneys and liver, with mutations in this gene associated with various kidney and liver disorders. Understanding the function and regulation of Nephrocystin-3 is essential for developing therapies to treat these diseases effectively. By utilizing the Nephrocystin-3 Polyclonal Antibody, researchers can further elucidate the mechanisms underlying these conditions and develop targeted treatments for patients in need.
Product Name:
NPHP3 Rabbit Polyclonal Antibody
SKU:
CAB8580
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human NPHP3 (NP_694972.3).
This gene encodes a protein containing a coiled-coil (CC) domain, a tubulin-tyrosine ligase (TTL) domain, and a tetratrico peptide repeat (TPR) domain. The encoded protein interacts with nephrocystin, it is required for normal ciliary development, and it functions in renal tubular development. Mutations in this gene are associated with nephronophthisis type 3, and also with renal-hepatic-pancreatic dysplasia, and Meckel syndrome type 7. Naturally occurring read-through transcripts exist between this gene and the downstream ACAD11 (acyl-CoA dehydrogenase family, member 11) gene.
Purification Method:
Affinity purification
Gene ID:
27031
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NPHP3 antibody (CAB8580) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
