The NeuroD1 Monoclonal Antibody (CAB12020) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. RRID Gene ID 4760 Swiss Prot Synonym T2D; BETA2; BHF-1; MODY6; NEUROD; bHLHa3; NeuroD1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
NeuroD1 Monoclonal Antibody
SKU:
CAB12020
Size:
100μL, 20μL
Reactivity:
Mouse
Clone Number:
ARC0483
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T2D, BETA2, BHF-1, MODY6, NEUROD, bHLHa3, NeuroD1
Positive Sample:
Mouse brain
Cellular Localization:
Cytoplasm, Nucleoplasm, Nucleus.
Calculated MW:
40kDa
Observed MW:
49kDa
This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. RRID Gene ID 4760 Swiss Prot Synonym T2D; BETA2; BHF-1; MODY6; NEUROD; bHLHa3; NeuroD1
Purification Method:
Affinity purification
Gene ID:
4760
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse brain, using NeuroD1 Rabbit mAb (CAB12020) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
