The NFAT2 Monoclonal Antibody (CAB19597) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it an excellent choice for Western blot and immunohistochemistry applications.NFAT2, also known as Nuclear Factor of Activated T cells 2, plays a crucial role in regulating gene expression in immune cells, particularly T cells. It is a key player in immune responses, influencing processes such as cytokine production and cell proliferation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NFAT2 Monoclonal Antibody
SKU:
CAB19597
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0076
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NFAT2, NFATc, NF-ATC, NF-ATc1.2
Positive Sample:
U-87MG, K-562, Jurkat, 293T
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
101kDa
Observed MW:
70-140kDa
The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
Purification Method
Affinity purification
Gene ID
4772
RRID
AB_2862690
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NFAT2 Rabbit mAb (CAB19597) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
