The NFU1 Antibody (CAB7097) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. RRID AB_2767652 Gene ID 27247 Swiss Prot Synonym Nfu; NifU; HIRIP; MMDFS; MMDS1; NIFUC; CGI-33; HIRIP5; NFU1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NFU1 Antibody
SKU:
CAB7097
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
BT-474, SW480, THP-1, U-937, Mouse liver, Mouse heart, Mouse skeletal muscle, Rat liver, Rat heart
Cellular Localization:
Cytoplasm, Mitochondrion, Cytosol.
Calculated MW:
28kDa
Observed MW:
25-28kDa
This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. RRID AB_2767652 Gene ID 27247 Swiss Prot Synonym Nfu; NifU; HIRIP; MMDFS; MMDS1; NIFUC; CGI-33; HIRIP5; NFU1
Purification Method:
Affinity purification
Gene ID:
27247
RRID:
AB_2767652
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NFU1 Rabbit pAb (CAB7097) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
