The NFYC Antibody (CAB9832) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications.NFYC is involved in a variety of cellular processes, including cell proliferation, differentiation, and apoptosis. By targeting the NFYC protein, this antibody allows for the detection and analysis of NFYC expression in different cell types, making it ideal for studies in molecular biology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
NFYC Antibody
SKU:
CAB9832
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HSM, CBFC, HAP5, CBF-C, NF-YC, H1TF2A, NFYC
Positive Sample:
Rat thymus
Cellular Localization:
Nucleus.
Calculated MW:
50kDa
Observed MW:
50kDa
This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4802
RRID
AB_2770580
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat thymus, using NFYC Rabbit pAb (CAB9832) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
