The NKX2-1 Monoclonal Antibody (CAB22247) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using innovative monoclonal antibody technology, is highly specific to NKX2-1 and has been validated for use in various research applications, including immunohistochemistry and Western blotting.NKX2-1 is a transcription factor that plays a crucial role in lung development and function, as well as in the regulation of thyroid and ventral forebrain development. Dysregulation of NKX2-1 has been implicated in various diseases, including lung cancer and thyroid disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NKX2-1 Monoclonal Antibody
SKU:
CAB22247
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC51284
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription.
Purification Method
Affinity purification
Gene ID
7080
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NKX2-1 Rabbit mAb (CAB22247) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Rat brain Exposure time: 45s.
Western blot analysis of various lysates using NKX2-1 Rabbit mAb (CAB22247) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): HeLa,A-549,Mouse Brain. Exposure time: 5s.
