The NLRP3 Monoclonal Antibody (CAB24294) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in mice, is highly specific to the NLRP3 protein and is validated for use in immunofluorescence and immunohistochemistry applications. By targeting NLRP3, researchers can investigate the role of this protein in inflammatory pathways, making it ideal for studies on inflammatory diseases, autoimmune disorders, and metabolic conditions.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
NLRP3 Monoclonal Antibody
SKU:
CAB24294
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC62768
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables DNA-binding transcription factor binding activity and sequence-specific DNA binding activity. Involved in several processes, including positive regulation of T-helper cell differentiation; positive regulation of cytokine production; and response to bacterium. Acts upstream of or within several processes, including NLRP3 inflammasome complex assembly; activation of cysteine-type endopeptidase activity involved in apoptotic process; and defense response to virus. Located in cytoplasm and nucleus. Part of NLRP3 inflammasome complex. Is expressed in central nervous system and retina. Used to study CINCA Syndrome; familial cold autoinflammatory syndrome 1; and non-alcoholic fatty liver disease. Human ortholog(s) of this gene implicated in CINCA Syndrome; Muckle-Wells syndrome; autosomal dominant nonsyndromic deafness 34; familial cold autoinflammatory syndrome 1; and urticaria. Orthologous to human NLRP3 (NLR family pyrin domain containing 3).
Purification Method
Affinity purification
Gene ID
216799
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from RAW 264.7 cells, using NLRP3 Rabbit mAb (CAB24294) at 1:1000 dilution. Raw264. 7 cells were treated with LPS (1 μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Confocal imaging of RAW 264.7 cells (treated with LPS) and RAW 264.7 cells (untreated) using NLRP3 Rabbit mAb (CAB24294, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
