The NNMT Monoclonal Antibody (CAB23786) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using hybridoma technology, is highly specific to NNMT and suitable for various applications including immunofluorescence, immunohistochemistry, and Western blot.NNMT plays a key role in the regulation of cellular energy metabolism, lipid metabolism, and inflammation, making it a potential target for therapeutic interventions in metabolic disorders, cancer, and neurodegenerative diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NNMT Monoclonal Antibody
SKU:
CAB23786
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC61849
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NNMT, nicotinamide N-methyltransferase
Positive Sample:
Caki-1, SK-OV-3, 786-O, HeLa
Cellular Localization:
Cytoplasm.
Calculated MW:
29kDa
Observed MW:
28kDa
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.
Purification Method
Affinity purification
Gene ID
4837
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NNMT Rabbit mAb (CAB23786) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): PANC-1. Exposure time: 20 s.
