The NNMT Monoclonal Antibody (CAB23786) is a high-quality antibody developed for reliable detection and analysis of target proteins. N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NNMT Monoclonal Antibody
SKU:
CAB23786
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC61849
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NNMT, nicotinamide N-methyltransferase
Positive Sample:
Caki-1, SK-OV-3, 786-O, HeLa
Cellular Localization:
Cytoplasm.
Calculated MW:
29kDa
Observed MW:
28 kDa
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.
Purification Method
Affinity purification
Gene ID
4837
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NNMT Rabbit mAb (CAB23786) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): PANC-1. Exposure time: 20 s.
