The iNOS Antibody (CAB0312) is a high-quality antibody developed for reliable detection and analysis of target proteins. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. RRID AB_2757120 Gene ID 4843 Swiss Prot Synonym NOS; INOS; NOS2A; HEP-NOS; iNOS
This antibody is validated for use in IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
iNOS Antibody
SKU:
CAB0312
Size:
100μL, 20μL
Reactivity:
Human, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
IF/ICCELISA
Recommended Dilution:
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NOS, INOS, NOS2A, HEP-NOS, iNOS
Positive Sample:
-
Cellular Localization:
Cortical Cytoskeleton, Cytoplasm, Cytosol, Nucleoplasm, Nucleus, Perinuclear Region Of Cytoplasm, Peroxisomal Matrix, Peroxisome, Plasma Membrane.
Calculated MW:
131kDa
Observed MW:
Refer to figures
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. RRID AB_2757120 Gene ID 4843 Swiss Prot Synonym NOS; INOS; NOS2A; HEP-NOS; iNOS
Purification Method:
Affinity purification
Gene ID:
4843
RRID:
AB_2757120
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Caco-2 cells using iNOS Rabbit pAb (CAB0312) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
