The NOSIP Antibody (CAB10024) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the NOSIP protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an ideal choice for studies in biochemistry and cell biology.NOSIP, also known as nitric oxide synthase-interacting protein, is involved in the regulation of NO production, a crucial mediator of various physiological processes including vasodilation and neurotransmission.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NOSIP Antibody
SKU:
CAB10024
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CGI-25, NOSIP
Positive Sample:
A-549, B cells, 293T
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
33kDa
Observed MW:
35kDa
The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein.
Purification Method
Affinity purification
Gene ID
51070
RRID
AB_2757544
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NOSIP Rabbit pAb (CAB10024) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
