The NPAS2 Antibody (CAB16930) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
NPAS2 Antibody
SKU:
CAB16930
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MOP4, PASD4, bHLHe9, NPAS2
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Cytosol, Nucleoplasm, Nucleus.
Calculated MW:
92kDa
Observed MW:
75kDa
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain.
Purification Method
Affinity purification
Gene ID
4862
RRID
AB_2770626
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NPAS2 Rabbit pAb (CAB16930) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of C6 cells using NPAS2 Rabbit pAb (CAB16930) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
