The NPAS2 Antibody (CAB16930) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in applications such as Western blotting. By binding specifically to the NPAS2 protein, this antibody enables the detection and analysis of NPAS2 in various cell types, making it ideal for studies in chronobiology and neuroscience.NPAS2 is a critical component of the molecular clock that governs daily physiological processes, including sleep-wake cycles and metabolic regulation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
NPAS2 Antibody
SKU:
CAB16930
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MOP4, PASD4, bHLHe9, NPAS2
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Cytosol, Nucleoplasm, Nucleus.
Calculated MW:
92kDa
Observed MW:
75kDa
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain.
Purification Method
Affinity purification
Gene ID
4862
RRID
AB_2770626
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NPAS2 Rabbit pAb (CAB16930) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of C6 cells using NPAS2 Rabbit pAb (CAB16930) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
