The NPHS2 Antibody (CAB17337) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By selectively binding to NPHS2, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for studies in nephrology and renal diseases.NPHS2, also known as podocin, is a crucial component of the kidney filtration barrier and plays a significant role in maintaining normal kidney function. Mutations in the NPHS2 gene have been linked to hereditary nephrotic syndrome, a severe kidney disorder characterized by proteinuria and edema.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NPHS2 Antibody
SKU:
CAB17337
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PDCN, SRN1, NPHS2
Positive Sample:
NTERA-2, Rat spleen
Cellular Localization:
Cell-Cell Junction, Cytoplasmic Side Of Plasma Membrane, Endoplasmic Reticulum, Extracellular Exosome, Plasma Membrane, Slit Diaphragm.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
7827
RRID
AB_2770633
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using NPHS2 Rabbit pAb (CAB17337) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
