The NR4A3 Polyclonal Antibody (PAC022488) is a valuable tool for researchers studying NR4A3, a nuclear receptor protein involved in various cellular processes, including inflammation and metabolism. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the NR4A3 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component in studies related to immunology and metabolic diseases.
NR4A3, also known as NOR-1, is a transcription factor that plays a crucial role in the regulation of gene expression related to metabolism, inflammation, and cell proliferation. Its involvement in these pathways makes NR4A3 a significant target for research investigating diseases such as diabetes, obesity, and cardiovascular disorders. Understanding the function of NR4A3 can provide valuable insights for developing targeted therapies and interventions to address these conditions effectively.
Antibody Name:
NR4A3 Antibody (PACO22488)
Antibody SKU:
PACO22488
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human NR4A3.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from COLO cells, using NR4A3 antibody.
Background:
Binds to the B1A response-element.
Synonyms:
Nuclear receptor subfamily 4 group A member 3; Nuclear hormone receptor NOR-1; Neuron-derived orphan receptor 1; Mitogen-induced nuclear orphan receptor;
UniProt Protein Function:
NR4A3: Binds to the B1A response-element. Defects in NR4A3 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving NR4A3 is found in patients with Erwing sarcoma. Translocation t(9;22)(q22-31;q11-12) with EWSR1. A chromosomal aberration involving NR4A3 is a cause of a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with TAF2N. Belongs to the nuclear hormone receptor family. NR4 subfamily. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Nuclear receptor; DNA-binding; OncoproteinChromosomal Location of Human Ortholog: 9q22Cellular Component: nucleoplasmMolecular Function: protein binding; protein homodimerization activity; protein kinase binding; thyroid hormone receptor activityBiological Process: cellular respiration; fat cell differentiation; gastrulation; mast cell degranulation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation; positive regulation of fatty acid oxidation; positive regulation of mast cell cytokine production; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; pyruvate oxidation; regulation of smooth muscle cell proliferation; transcription initiation from RNA polymerase II promoterDisease: Chondrosarcoma, Extraskeletal Myxoid
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcriptional activator. The protein can efficiently bind the NGFI-B Response Element (NBRE). Three different versions of extraskeletal myxoid chondrosarcomas (EMCs) are the result of reciprocal translocations between this gene and other genes. The translocation breakpoints are associated with Nuclear Receptor Subfamily 4, Group A, Member 3 (on chromosome 9) and either Ewing Sarcome Breakpoint Region 1 (on chromosome 22), RNA Polymerase II, TATA Box-Binding Protein-Associated Factor, 68-KD (on chromosome 17), or Transcription factor 12 (on chromosome 15). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
