The NRF2 Monoclonal Antibody (CAB21176) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated in mice, is specifically designed for use in immunohistochemistry and immunofluorescence applications, providing high specificity and sensitivity for detecting NRF2 expression in various tissue samples.NRF2 is a master regulator of antioxidant response genes, playing a key role in protecting cells from oxidative damage and promoting cell survival under stress conditions. Dysregulation of NRF2 signaling has been implicated in a variety of diseases, including cancer, neurodegenerative disorders, and metabolic syndromes.
This antibody is validated for use in WB, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NRF2 Monoclonal Antibody
SKU:
CAB21176
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC50393
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
Purification Method
Affinity purification
Gene ID
4780
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] NRF2 Rabbit mAb (CAB21176) at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 and C6 cells were treated with MG132(50 μM) at 37℃ for 90 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Western blot analysis of lysates from wild type (WT) and NRF2 knockout (KO) HeLa cells using [KO Validated] NRF2 Rabbit mAb (CAB21176) at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated with MG132(50 μM) at 37℃ for 90 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
