The NXF1 Monoclonal Antibody (CAB3488) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the NXF1 protein, it enables the detection and analysis of this key component in mRNA transport processes within the cell.NXF1 plays a crucial role in gene expression by facilitating the export of mature mRNA from the nucleus to the cytoplasm, where it can be translated into proteins. Dysregulation of NXF1 function has been implicated in various diseases, such as cancer and neurological disorders, highlighting its importance in cellular processes.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NXF1 Monoclonal Antibody
SKU:
CAB3488
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2013
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TAP, MEX67, NXF1
Positive Sample:
293T, 293F, A549, THP-1
Cellular Localization:
Cytoplasm, Nucleus, Nucleus Speckle, Nucleoplasm.
Calculated MW:
70kDa
Observed MW:
72kDa
This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10482
RRID
AB_2863070
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NXF1 Rabbit mAb (CAB3488) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation of NXF1 from 300 µg extracts of 293F cells was performed using 3 µg of NXF1 Rabbit mAb (CAB3488). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using NXF1 Rabbit mAb (CAB3488) at a dilution of 1:500.
