The OGG1 Monoclonal Antibody (CAB4997) is a high-quality antibody developed for reliable detection and analysis of target proteins. OGG1, a key enzyme involved in the base excision repair pathway, plays a crucial role in maintaining genomic integrity by repairing oxidative DNA damage caused by reactive oxygen species. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting OGG1 protein levels in various experimental settings.Validated for use in Western blot and immunohistochemistry applications, the OGG1 Rabbit Monoclonal Antibody enables researchers to analyze OGG1 expression and localization in different cell types and tissues.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
OGG1 Monoclonal Antibody
SKU:
CAB4997
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC1233
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.
Purification Method
Affinity purification
Gene ID
4968
RRID
AB_2863408
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using OGG1 Rabbit mAb (CAB4997) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using OGG1 Rabbit mAb (CAB4997) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
