The OTC Antibody (CAB9834) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed for use in Western blot applications and has been validated for its reactivity with human samples.OTC, or Ornithine Transcarbamylase, plays a crucial role in the detoxification of ammonia in the body by converting ornithine and carbamoyl phosphate into citrulline. Dysregulation of OTC can lead to hyperammonemia, a serious condition that can result in neurological damage or death.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
OTC Antibody
SKU:
CAB9834
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OCTD, OTC1, OTCD, OTCase, OTC
Positive Sample:
293T transfected with OTC, Mouse liver, Rat liver
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
40kDa
Observed MW:
40kDa/42kDa
This nuclear gene encodes a mitochondrial matrix enzyme. The encoded protein is involved in the urea cycle which functions to detoxify ammonia into urea for excretion. Mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia.
Purification Method
Affinity purification
Gene ID
5009
RRID
AB_2770748
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with OTC using OTC Rabbit pAb (CAB9834) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates, using OTC Rabbit pAb (CAB9834) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
