The P Glycoprotein Monoclonal Antibody (CAB19093) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, developed using advanced monoclonal antibody technology, specifically targets P-Glycoprotein and is highly reactive with human samples. Validated for use in various applications, including Western blot and immunohistochemistry, this antibody enables accurate detection and analysis of P-Glycoprotein in different cell types.P-Glycoprotein, a member of the ATP-binding cassette (ABC) transporter family, plays a crucial role in drug efflux and resistance mechanisms in cancer cells. Its overexpression in cancer cells can lead to decreased efficacy of chemotherapy drugs, posing a significant challenge in cancer treatment.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
P Glycoprotein Monoclonal Antibody
SKU:
CAB19093
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0470
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170, p-170, P Glycoprotein
Positive Sample:
293T, Mouse brain, C6, Rat brain, Hep G2
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
141kDa
Observed MW:
130-180kDa
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5243
RRID
AB_2862585
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] P Glycoprotein Rabbit mAb (CAB19093) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): MCF7 Exposure time: 30s.
Western blot analysis of various lysates using [KO Validated] P Glycoprotein Rabbit mAb (CAB19093) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
