The P4HA2 Monoclonal Antibody (CAB22150) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced using advanced technology, specifically targets P4HA2 in human samples and is suitable for use in various research applications, including immunohistochemistry and flow cytometry.P4HA2 plays a crucial role in the regulation of collagen synthesis, which is essential for tissue remodeling, wound healing, and tumor progression. By targeting P4HA2, researchers can gain insights into its functions and potential therapeutic implications in diseases such as cancer, fibrosis, and tissue fibrosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
P4HA2 Monoclonal Antibody
SKU:
CAB22150
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC54912
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method
Affinity purification
Gene ID
8974
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using P4HA2 Rabbit mAb (CAB22150) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): HL-60. Exposure time: 90 s.
