The Pan DiMethyl-lysine Antibody (CAB18296) is a high-quality antibody developed for reliable detection and analysis of target proteins. The lysine methylation chemical reaction is the reversible addition of one, two or three methyl groups to the ε-nitrogen of a lysine side chain, forming monomethylated, dimethylated and trimethylated derivatives (referred to here as ‘Kme1’, ‘Kme2’ and ‘Kme3’).
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Pan DiMethyl-lysine Antibody
SKU:
CAB18296
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
NIH/3T3, C6, Mouse liver, Rat liver, HeLa, MCF7
Observed MW:
17-48kDa
The lysine methylation chemical reaction is the reversible addition of one, two or three methyl groups to the ε-nitrogen of a lysine side chain, forming monomethylated, dimethylated and trimethylated derivatives (referred to here as ‘Kme1’, ‘Kme2’ and ‘Kme3’).
Purification Method
Affinity purification
RRID
AB_2862069
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Pan DiMethyl-lysine Rabbit pAb (CAB18296) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
