The pan-methyl-Histidine Polyclonal Antibody (CAB17948) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By specifically binding to pan-methyl histidine residues, this antibody allows for the detection and analysis of proteins modified by this post-translational modification.Histidine methylation is a crucial regulatory mechanism in various cellular processes, including signal transduction, protein-protein interactions, and gene expression.
This antibody is validated for use in WB, ELISA, DB applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
pan-methyl-Histidine Polyclonal Antibody
SKU:
CAB17948
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISADB
Recommended Dilution:
WB
1:500 - 1:1000
DB
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HeLa, HepG2, A-549, NIH/3T3, C6, Mouse brain, Mouse liver, Rat brain, Rat liver
Observed MW:
10-100kDa
Purification Method
Affinity purification
RRID
AB_2770786
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using pan-methyl-Histidine Rabbit pAb (CAB17948) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 300s.
