The Pan Mono-Methyl lysine Antibody (CAB18293) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity in detecting mono-methylated lysine residues in various protein samples, with a particular affinity for human samples.The antibody is validated for use in a variety of applications, including Western blotting, immunoprecipitation, and immunofluorescence, allowing for the detection and analysis of mono-methylated lysine proteins in different cell types and tissues. Its versatility makes it ideal for studies in epigenetics, chromatin remodeling, and gene expression regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Pan Mono-Methyl lysine Antibody
SKU:
CAB18293
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HeLa, MCF7, NIH/3T3, C6, Mouse liver, Rat liver
Observed MW:
17-90kDa
Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs).
Purification Method
Affinity purification
RRID
AB_2862066
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Pan Mono-Methyl lysine Rabbit pAb (CAB18293) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Western blot analysis of various lysates using Pan Mono-Methyl lysine Rabbit pAb (CAB18293) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
