The pan-Symmetric-Di-Methyl Arginine Antibody (CAB18261) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for detecting proteins with symmetric di-methyl arginine modifications, providing accurate and reliable results in various applications.Symmetric di-methyl arginine modifications play a crucial role in regulating protein function, protein-protein interactions, and gene expression. By targeting these modifications, researchers can gain insights into the mechanisms underlying various biological processes, such as chromatin remodeling, RNA processing, and cell signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
pan-Symmetric-Di-Methyl Arginine Antibody
SKU:
CAB18261
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HeLa, MCF7, NIH/3T3, C6, Mouse liver, Rat liver
Observed MW:
22-70kDa
The methylation of arginine residues in proteins is a post-translational modification that contributes to a wide range of biological processes. Many cytokines involved in T cell development and activation utilize the common cytokine receptor γ-chain (γc) and the kinase JAK3 for signal transduction.
Purification Method
Affinity purification
RRID
AB_2862036
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using pan-Symmetric-Di-Methyl Arginine Rabbit pAb (CAB18261) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Dot-blot analysis of all sorts of methylation peptides using pan Symmetric-DiMethyl-Arginine antibody (CAB18261) at 1:1000 dilution.
