The PANX3 Antibody (PAC059285) is a polyclonal antibody designed for research involving PANX3, a member of the Pannexin family of channel proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.PANX3 is a transmembrane protein involved in various physiological processes, including cell communication, calcium signaling, and development of skin and bone tissues. Its expression has been linked to conditions such as osteoarthritis, skeletal dysplasia, and skin disorders. The PANX3 Antibody enables researchers to detect and analyze the presence of PANX3 protein in different cell types, making it a valuable tool for studies in the fields of skeletal biology, dermatology, and musculoskeletal disorders.
By understanding the role of PANX3 in these biological processes and diseases, researchers can uncover potential therapeutic targets for developing treatments and interventions to address conditions associated with PANX3 dysfunction. This antibody is a valuable resource for scientists conducting research in areas related to skeletal health, skin disorders, and musculoskeletal diseases.
Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate. All lanes: PANX3 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa.
Immunofluorescence staining of HepG2 cells with PACO59285 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Structural component of the gap junctions and the hemichannels.
Synonyms:
Pannexin-3, PANX3
UniProt Protein Function:
PANX3: Structural component of the gap junctions and the hemichannels. Belongs to the pannexin family.Protein type: Membrane protein, integral; Cell adhesion; Membrane protein, multi-passChromosomal Location of Human Ortholog: 11q24.2Cellular Component: endomembrane system; plasma membraneMolecular Function: gap junction hemi-channel activity; wide pore channel activityBiological Process: cation transport; cell-cell signaling
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the innexin family. Innexin family members are known to be the structural components of gap junctions. [provided by RefSeq, Jul 2008]
