The PBK Polyclonal Antibody (CAB21569) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is specifically designed for use in Western blot applications and is highly reactive with human samples. By targeting the CD300A protein, researchers can detect and analyze its expression in various cell types, making it ideal for studies in immunology and cancer research.CD300A, also known as an immune inhibitory receptor, plays a crucial role in immune homeostasis by regulating inflammation and allergic reactions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PBK Polyclonal Antibody
SKU:
CAB21569
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SPK, CT84, TOPK, HEL164, Nori-3, PBK
Positive Sample:
Mouse spleen, Rat testis, HeLa, 293T
Cellular Localization:
Nucleus.
Calculated MW:
36kDa
Observed MW:
36kDa
This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
55872
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PBK Rabbit pAb (CAB21569) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from wild type (WT) and PBK knockout (KO) 293T cells using PBK Rabbit pAb (CAB21569) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
