The PCGF1 Antibody (CAB6338) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the PCGF1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in cancer biology and developmental biology.PCGF1 is a member of the polycomb group (PcG) protein family, which plays a crucial role in epigenetic regulation of gene expression.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PCGF1 Antibody
SKU:
CAB6338
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NSPC1, RNF68, RNF3A-2, 2010002K04Rik, PCGF1
Positive Sample:
HeLa, A-549, MCF7, Mouse testis, Mouse brain, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
30kDa
Observed MW:
35kDa
PCGF1 is a mammalian homolog of the Drosophila polycomb group genes, which act as transcriptional repressors to regulate anterior-posterior patterning in early embryonic development (Nunes et al., 2001 [PubMed 11287196]). See also PCGF2 (MIM 600346).
Purification Method
Affinity purification
Gene ID
84759
RRID
AB_2766941
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PCGF1 Rabbit pAb (CAB6338) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of U-2OS cells using PCGF1 Rabbit pAb (CAB6338) at dilution of 1:100. Blue: DAPI for nuclear staining.
