The PEPCK/PCK2 Antibody (CAB8446) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. By binding specifically to the PCK2 protein, this antibody enables reliable detection and analysis in a variety of cell types, making it an excellent choice for studies in metabolic regulation, cancer metabolism, and metabolic diseases.PCK2, also known as phosphoenolpyruvate carboxykinase 2, plays a crucial role in gluconeogenesis and glyceroneogenesis, processes essential for maintaining glucose homeostasis and energy balance in the body.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PEPCK/PCK2 Antibody
SKU:
CAB8446
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PEPCK, PEPCK2, PEPCK-M, PEPCK/PCK2
Positive Sample:
Hep G2, Mouse brain, Rat brain
Cellular Localization:
Mitochondrion.
Calculated MW:
71kDa
Observed MW:
69kDa
This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
5106
RRID
AB_2770812
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Hep G2 cells using PEPCK/PCK2 Rabbit pAb (CAB8446) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates using PEPCK/PCK2 Rabbit pAb (CAB8446) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
