The PCPE-1 Monoclonal Antibody (CAB24732) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, generated using hybridoma technology, offers high specificity and sensitivity in detecting PCPE-1 in various biological samples.PCPE-1, also known as procollagen C-endopeptidase enhancer 1, plays a crucial role in the processing of procollagen, a key step in collagen production. Dysregulation of PCPE-1 has been linked to various collagen-related disorders, making it an important target for research in the fields of connective tissue disorders and fibrosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PCPE-1 Monoclonal Antibody
SKU:
CAB24732
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC63882
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PCPE, PCPE1, PCPE-1
Positive Sample:
U-2 OS
Cellular Localization:
Secreted.
Calculated MW:
48kDa
Observed MW:
48kDa
Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.
Purification Method
Affinity purification
Gene ID
5118
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using PCPE-1 Rabbit mAb (CAB24732) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC):THP-1. Exposure time: 30 s.
Western blot analysis of lysates from Rat testis using PCPE-1 Rabbit mAb (CAB24732) at 1:3000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
