The PD-L1 Mouse Monoclonal Antibody (CAB18103) is a valuable tool for research involving PD-L1, a key immune checkpoint protein involved in immune regulation and cancer progression. This antibody, derived from mouse cells, is highly specific for PD-L1 and is validated for use in various applications, including immunohistochemistry and flow cytometry.PD-L1, also known as programmed death-ligand 1, plays a crucial role in suppressing the immune response by binding to its receptor PD-1 on T-cells, leading to T-cell exhaustion and immune evasion by cancer cells. The expression of PD-L1 in tumor cells has been linked to resistance to immunotherapy and poor prognosis in cancer patients.
By utilizing the PD-L1 Mouse Monoclonal Antibody, researchers can explore the expression and function of PD-L1 in cancer and other disease contexts, paving the way for the development of novel therapeutic strategies targeting the PD-L1/PD-1 immune checkpoint pathway. This antibody is a valuable resource for studies in immunology, oncology, and immuno-oncology, offering insights into potential treatment options for patients with PD-L1-positive tumors.
Product Name:
PD-L1/CD274 Mouse Monoclonal Antibody
SKU:
CAB18103
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant protein of human PD‐L1 extracellular domain.
Cell membrane, Endomembrane system, Single-pass type I membrane protein
Calculated MW:
33kDa
Observed MW:
40-50kDa
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
29126
Clone Number:
ARC5110-01
Storage Buffer:
Store at 4℃. Avoid freeze / thaw cycles.Tris Buffer, pH7.3-7.7, with 1% BSA and <0.1% Sodium azide.
Western blot analysis of lysates from Raji cells, using PD-L1/CD274 Rabbit mAb (CAB18103) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
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