The PDCD4 Monoclonal Antibody (CAB9068) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PDCD4 Monoclonal Antibody
SKU:
CAB9068
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1398
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IHC-P
1:100 - 1:400
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
H731, PDCD4
Positive Sample:
HeLa, 293T, U-937, Mouse liver, Mouse thymus
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
52kDa
Observed MW:
52kDa
This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
27250
RRID
AB_2863650
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PDCD4 Rabbit mAb (CAB9068) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
Immunohistochemistry analysis of paraffin-embedded Rat lung using PDCD4 Rabbit mAb (CAB9068) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
