The PDK3 Antibody (CAB12480) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically recognizes human PDK3 and is validated for use in Western blot applications. By binding to the PDK3 protein, this antibody facilitates the detection and analysis of PDK3 levels in various cell types, making it an essential component for studies in metabolism, cancer research, and other related fields.PDK3 plays a crucial role in metabolic processes, particularly in the regulation of glucose metabolism and mitochondrial function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PDK3 Antibody
SKU:
CAB12480
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CMTX6, GS1-358P8.4, PDK3
Positive Sample:
SH-SY5Y
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
47kDa
Observed MW:
47kDa
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5165
RRID
AB_2759323
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from SH-SY5Y cells, using PDK3 Rabbit pAb (CAB12480) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
