The PE-Cyanine7 Anti-Human CD274 (PD-L1) Antibody (29E.2A3) is a cutting-edge tool for researchers delving into the complex realm of immune checkpoint regulation. This antibody, optimized for detecting human CD274 (PD-L1) protein, is essential for probing the mechanisms by which this immune checkpoint molecule modulates the immune response. By specifically targeting PD-L1—a key player in immune evasion and tolerance enforcement—the PE-Cyanine7 Anti-Human CD274 (PD-L1) Antibody (29E.2A3) enables precise visualization and quantification of PD-L1 expression in various cell types. This capability positions it as an invaluable asset in immunology and oncology research, where understanding PD-L1's role is crucial for advancing immunotherapeutic strategies for cancer treatment.
Harnessing the power of this highly reactive antibody, researchers can explore the intricate interplay between PD-L1 expression, immune evasion, and cancer progression. With its proven efficacy in applications such as flow cytometry and immunofluorescence, the PE-Cyanine7 Anti-Human CD274 (PD-L1) Antibody (29E.2A3) offers a versatile and reliable tool for unraveling the complexities of immune checkpoint modulation in health and disease.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
Q9NZQ7
Gene ID:
29126
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.
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