![PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Control [2A3] (AGEL2648)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/229313/602185/percpcyanine5.5-rat-igg2a-k-isotype-control-2a3-agel2648__64529.1706283904.jpg?c=2?imbypass=on "PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Control [2A3] (AGEL2648)")
Description
PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Control [2A3]
The PERCP/Cyanine5.5 Rat IgG2a Isotype Control (2A3) is a valuable tool for flow cytometry experiments requiring an isotype control antibody. This antibody, specifically designed for rat samples, serves as a crucial control in immunophenotyping studies by helping to distinguish non-specific background signal from specific antibody binding.Flow cytometry is a powerful technique used in immunology and cell biology research to analyze and quantify cell populations based on specific surface markers. The use of isotype controls, such as the PERCP/Cyanine5.5 Rat IgG2a Isotype Control (2A3), is essential for accurately interpreting flow cytometry data by providing a baseline for comparison with experimental antibodies targeting proteins of interest.
With its high specificity and low background binding, this isotype control antibody ensures reliable and reproducible flow cytometry results. Researchers can confidently use the PERCP/Cyanine5.5 Rat IgG2a Isotype Control (2A3) to validate staining protocols, set gating parameters, and standardize experimental conditions in their flow cytometry experiments. Its consistent performance makes it an indispensable component in immunophenotyping studies aimed at elucidating the complex interactions of different cell populations within biological systems.
| Product Name: | PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Control [2A3] (AGEL2648) |
| Product Code: | AGEL2648 |
| Clone No: | 2A3 |
| Host Species: | Rat |
| Applications: | FCM |
| Isotype: | Rat IgG2a, κ |
| Conjugation: | PerCP/Cyanine5.5 |
| Form: | Liquid |
| Fluorescent Label: | PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter). |
| Recommended Use: | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
| Storage Buffer: | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
| Stability & Storage: | Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
| Shipping: | Biological ice pack at 4°C |
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