The PGD2 (Prostaglandin D2) ELISA Kit is specifically designed for the precise measurement of PGD2 levels in biological samples such as serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for various research applications.Prostaglandin D2 is a lipid mediator that plays a key role in inflammatory responses, allergic reactions, and immune regulation. Abnormal levels of PGD2 have been linked to various diseases such as asthma, allergic rhinitis, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its advanced technology and user-friendly protocols, the PGD2 ELISA Kit from Assay Genie is a valuable tool for researchers looking to explore the role of PGD2 in health and disease. Get accurate and reliable data with this innovative ELISA kit.
Product Name:
PGD2 (Prostaglandin D2) ELISA Kit
SKU:
UNES00043
Target:
PGD2 (Prostaglandin D2) (UNES00043)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGD2. During the reaction, PGD2 in the sample or standard competes with a fixed amount of PGD2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGD2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGD2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
97-110
85-99
Average (%)
102
104
91
1:4
Range (%)
86-96
90-105
99-115
Average (%)
91
96
106
1:8
Range (%)
85-97
88-103
93-107
Average (%)
90
95
99
1:16
Range (%)
89-103
93-105
95-108
Average (%)
96
98
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.5
101.6
404.9
46.3
103.6
364.6
Standard deviation
2.6
5.9
12.1
3.1
5.9
13.1
C V (%)
5.25
5.81
2.99
6.7
5.69
3.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of PGD2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes PGD2 in samples. No significant cross-reactivity or interference between PGD2 and analogues was observed.
