PharmaGenie ELISA Kits Protocol
About PharmaGenie ELISA Kits
PharmaGenie ELISA Kits from Assay Genie are highly validated ELISA Kits designed to exceed the exacting needs of biopharma R&D scientists. Focusing on high quality antibody pairs & extensively optimized reagents that have been validated according to ISO quality systems.
Features
- Highly validated pre-coated 96 well plates
- High specificity & down to picogram sensitivity for many analytes
- Over 2,000 kits covering 15+ species
- Compatible with plasma, serum, cell culture media, lysates & clarified body fluids
- Reproducible and reliable
PharmaGenie ELISA Kits with Controls Protocol Step-by-Step
Figure 1: Schematic of a PharmaGenie ELISA with controls protocol.
| Step | Procedure |
|
1. |
Add 100µl of each standard, sample, control and zero (standard diluent) in duplicate to appropriate number of wells |
|
2. |
Add 50µl of diluted biotinylated detection antibody to all wells |
|
3. |
Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 1 hour |
|
4. |
Remove the cover and wash the plate as follows: |
|
5. |
Add 100µl of Streptavidin-HRP solution into all wells |
|
6. |
Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 30 min |
|
7. |
Repeat wash step 4 |
|
8. |
Add 100µl of ready-to-use TMB Substrate Solution into all wells |
|
9. |
Incubate in the dark for 10-20 minutes* at room temperature. Avoid direct exposure to light by wrapping the plate in aluminium foil |
|
10. |
Add 100µl of H2SO4: Stop Reagent into all wells |
|
11. |
Read the absorbance value of each well (immediately after step 10.) on a spectrophotometer using 450 nm as the primary wavelength and optionally 630 nm as the reference wave length (610 nm to 650 nm is acceptable). |
Note: See technical manual for specific PharmaGenie ELISA kits for full details.
Related Information
*A subset of PharmaGenie ELISA kits are available at 2 for €499, however, some kits maybe be 2 for €599.