The Phospho-ACLY-S455 Antibody (CABP0779) is a high-quality antibody developed for reliable detection and analysis of target proteins. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-ACLY-S455 Antibody
SKU:
CABP0779
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB
1:100 - 1:500
IHC-P
1:50 - 1:100
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACL, ATPCL, CLATP, Phospho-ACLY-S455
Positive Sample:
C6 treated with Insulin, NIH/3T3 treated with Insulin, HeLa treated with Insulin
Cellular Localization:
Cytoplasm.
Calculated MW:
121kDa
Observed MW:
125kDa
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
Purification Method
Affinity purification
Gene ID
47
RRID
AB_2770895
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C6 cells using Phospho-ACLY-S455 Rabbit pAb (CABP0779) at 1:400 dilution. C6 cells were treated with Insulin (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-ACLY-S455 Rabbit pAb (CABP0779) at 1:400 dilution. NIH/3T3 cells were treated with Insulin (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat pancreas using Phospho-ACLY-S455 Rabbit pAb (CABP0779) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-ACLY-S455 Rabbit pAb (CABP0779) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunoprecipitation analysis of 200 μg extracts of NIH/3T3 cells, using 3 μg Phospho-ACLY-S455 pAb (CABP0779). Western blot was performed from the immunoprecipitate using Phospho-ACLY-S455 pAb (CABP0779) at a dilution of 1:1000. NIH/3T3 cells were treated with Insulin (100 nM) at 37℃ for 10 minutes after serum-starvation overnight.
