The Phospho-AKT2 specific-S474 Polyclonal Antibody (CABP1403) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to the phosphorylated form of Akt2 at serine 474, enabling precise detection and quantification in Western blot applications.Akt2 is a member of the Akt family of serine/threonine protein kinases, known for their role in promoting cell proliferation and inhibiting apoptosis. Phosphorylation of Akt2 at serine 474 is crucial for its activation and downstream signaling, making this antibody essential for studying the regulation of this important pathway.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-AKT2 specific-S474 Polyclonal Antibody
SKU:
CABP1403
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
293F treated with Calyculin A, 293F treated with Calyculin A after transfected with AKT2
Cellular Localization:
Cell Membrane, Cytoplasm, Early Endosome, Nucleus, Peripheral Membrane Protein.
Calculated MW:
56kDa
Observed MW:
63kDa/90kDa
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.
Purification Method
Affinity purification
Gene ID
208
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit pAb (CABP1403) at 1:900 dilution. 293F cells were treated with Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit pAb (CABP1403) at 1:900 dilution. 293F cells,293F cells transfected with AKT3 and 293F cells transfected with AKT2 were treated with Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
