Description
Phospho-B-Raf-T401 Monoclonal Antibody (CABP1041)
The Phospho-B-Raf-T401 Monoclonal Antibody (CABP1041) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, specifically raised in rabbits, is highly specific and reactive with human samples, making it a valuable tool for Western blot applications.B-Raf is a key player in the MAPK/ERK signaling pathway, which is involved in cell proliferation, differentiation, and survival. Phosphorylation at Threonine 401 is a crucial regulatory event that modulates B-Raf activity and downstream signaling. The CABP1041 antibody enables researchers to specifically detect and analyze the phosphorylation status of B-Raf in various cell types, providing insights into its role in cancer, development, and other biological processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
| Product Name: | Phospho-B-Raf-T401 Monoclonal Antibody |
| SKU: | CABP1041 |
| Size: | 20μL, 100μL |
| Reactivity: | Rat |
| Clone Number: | ARC1587 |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | SATP P | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | NS7, B-raf, BRAF1, RAFB1, B-RAF1, BRAF-1, Phospho-B-Raf-T401 |
| Positive Sample: | C6 treated with PMA |
| Cellular Localization: | Cell Membrane, Cytoplasm, Nucleus. |
| Calculated MW: | 84kDa |
| Observed MW: | 85kDa |
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
| Purification Method | Affinity purification |
| Gene ID | 673 |
| RRID | AB_2863918 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from C6 cells, using Phospho-B-Raf-T401 Rabbit mAb (CABP1041) at 1:1000 dilution. C6 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
