The Phospho-EEF2K-S366 Antibody (CABP0845) is a high-quality antibody developed for reliable detection and analysis of target proteins. EEF2K is a key regulator of protein synthesis and cell growth, making it an important target for investigations into cancer, neurodegenerative diseases, and other conditions.This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically recognizing the phosphorylated form of EEF2K, researchers can gain crucial insights into the signaling pathways involved in protein translation and cell proliferation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-EEF2K-S366 Antibody
SKU:
CABP0845
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TLSG S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
eEF-2K, CaMKIII, HSU93850, Phospho-EEF2K-S366
Positive Sample:
HeLa, HeLa treated with PMA, NIH/3T3 treated with PMA, C6 treated with PMA
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
Purification Method
Affinity purification
Gene ID
29904
RRID
AB_2771037
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-EEF2K-S366 Rabbit pAb (CABP0845) at 1:1000 dilution. Hela cell lysate were treated with CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated with PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Western blot analysis of various lysates using Phospho-EEF2K-S366 Rabbit pAb (CABP0845) at 1:1000 dilution. NIH/3T3 and C6 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
