The Phospho-EGFR-Y1068 Antibody (CABP0301) is a high-quality antibody developed for reliable detection and analysis of target proteins. EGFR, or epidermal growth factor receptor, is a transmembrane protein that plays a key role in cell growth and proliferation. The phosphorylation of tyrosine residue 1068 on EGFR is known to be important for downstream signaling cascades involved in cell survival and proliferation.This antibody, raised in rabbits, is highly specific for the phosphorylated form of EGFR at tyrosine 1068 and has been validated for use in various applications, including Western blot and immunofluorescence.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-EGFR-Y1068 Antibody
SKU:
CABP0301
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PEYI N
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Membrane, Endoplasmic Reticulum Membrane, Endosome, Endosome Membrane, Golgi Apparatus Membrane, Nucleus Membrane, Nucleus, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
134kDa
Observed MW:
185kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method
Affinity purification
Gene ID
1956
RRID
AB_2771046
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-EGFR-Y1068 Rabbit pAb (CAB11351). HeLa cells were treated with EGF (100 ng/mL) at 37℃ for 30 minutes or treated with ATP(5 mM) at 30℃ for 1 hour after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
