The Phospho-eIF4EBP1-T70 Antibody (CABP0031) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to the phosphorylated form of eIF4EBP1 and is validated for use in Western blot applications.eIF4EBP1 is a crucial player in the control of translation initiation, and its phosphorylation at T70 is known to play a critical role in this process. By targeting the phosphorylated form of eIF4EBP1, researchers can gain insights into the regulation of protein synthesis and its impact on cellular processes such as growth, proliferation, and differentiation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-eIF4EBP1-T70 Antibody
SKU:
CABP0031
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TKTP P
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BP-1, 4EBP1, 4E-BP1, PHAS-I, Phospho-eIF4EBP1-T70
Positive Sample:
HeLa
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
13kDa
Observed MW:
17kDa
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method
Affinity purification
Gene ID
1978
RRID
AB_2771076
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-eIF4EBP1-T70 Rabbit pAb (CABP0031) at 1:2000 dilution. HeLa cells were treated by Insulin (100 nM) at 37℃ for 5 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-eIF4EBP1-T70 Rabbit pAb (CABP0031) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-eIF4EBP1-T70 Rabbit pAb (CABP0031) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
