The Phospho-ErbB4/HER4-Y1284 Antibody (CABP0034) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. RRID AB_2771096 Gene ID 2066 Swiss Prot Synonym HER4; ALS19; p180erbB4; Phospho-ErbB4/HER4-Y1284
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-ErbB4/HER4-Y1284 Antibody
SKU:
CABP0034
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HER4, ALS19, p180erbB4, Phospho-ErbB4/HER4-Y1284
Positive Sample:
MCF7 treated with IGF
Cellular Localization:
Cell Membrane, Mitochondrion, Nucleus, Single-Pass Type I Membrane Protein.
Calculated MW:
147kDa
Observed MW:
180kDa
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. RRID AB_2771096 Gene ID 2066 Swiss Prot Synonym HER4; ALS19; p180erbB4; Phospho-ErbB4/HER4-Y1284
Purification Method:
Affinity purification
Gene ID:
2066
RRID:
AB_2771096
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells using Phospho-ErbB4/HER4-Y1284 Rabbit pAb (CABP0034) at 1:700 dilution. MCF7 cells were treated with IGF (200 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 20 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
