Anti-Phospho-ERK1/ERK2 Antibody (CABP0472)

Antibody Name:	Anti-Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Antibody
Antibody SKU:	CABP0472
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, NIH/3T3, C6

Immunogen:	A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FLTE YVA
Gene ID:	5594/5595
Uniprot:	P28482/P27361
Cellular Location:
Calculated MW:	36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW:	42KDa/44KDa

	Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (CABP0472) at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded human colon using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (CABP0472) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (CABP0472) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (CABP0472) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Anti-Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Antibody (CABP0472). Western blot was performed from the immunoprecipitate using Anti-Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Antibody (CABP0472) at a dilition of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37'C for 10 minutes.

Synonyms:

MAPK1/MAPK3

Background:

This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]

Antibodies

Anti-ERK1 / ERK2 Antibody (CAB16686)

Anti-MAPK1/MAPK3 Antibody (CAB16736)

Anti-ERK1 / ERK2 Mouse Monoclonal Antibody (CAB10613)

Anti-ERK1/2 Antibody (CAB4782)

Anti-Phospho-ERK1-T202 + ERK2-T185 Antibody (CABP0485)