The Phospho-FADD-S194 Antibody (CABP1054) is a high-quality antibody developed for reliable detection and analysis of target proteins. FADD, or Fas-associated death domain protein, is a key mediator of apoptotic cell death and plays a crucial role in regulating cell survival and death.This antibody is raised in rabbits and is highly specific to phosphorylated FADD at serine 194. It is validated for use in various research applications, including Western blotting, immunohistochemistry, and immunofluorescence. By binding specifically to phospho-FADD (S194), researchers can accurately study the activation and function of FADD in different cell types and conditions, particularly in the context of cancer research and apoptosis regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-FADD-S194 Antibody
SKU:
CABP1054
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
AMSP M
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
Purification Method
Affinity purification
Gene ID
8772
RRID
AB_2863926
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-FADD-S194 Rabbit pAb (CAB5819). Hela cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour.HeLa cells were treated with nocodazole (50 ng/mL) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
