The Phospho-FAK-Y397 Monoclonal Antibody (CABP1447) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed by AssayGenie, specifically targets the phosphorylated form of FAK at the tyrosine 397 residue, which is critical for FAK activation and downstream signaling cascades.The Phospho-FAK (Y397) Monoclonal Antibody is highly specific and sensitive, making it ideal for use in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Its ability to detect phosphorylated FAK allows for the precise analysis of FAK activation in cell and tissue samples, providing insight into cell migration, proliferation, and survival mechanisms.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-FAK-Y397 Monoclonal Antibody
SKU:
CABP1447
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC62271
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
DDYA EI
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5747
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-549 cells, using Phospho-FAK-Y397 Rabbit mAb (CABP1447) at 1:4000 dilution. A-549 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
