The Phospho-Fyn-S21 Antibody (CABP0510) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the phosphorylated form of Fyn at Serine 21, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in signal transduction pathways and cancer research.Fyn is a member of the Src family of kinases and is known to play a role in cell growth, differentiation, and immune responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Fyn-S21 Antibody
SKU:
CABP0510
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
DGSL N
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SLK, SYN, p59-FYN, Phospho-Fyn-S21
Positive Sample:
A-431 treated with 10% FBS
Cellular Localization:
Cell Membrane, Cytoplasm, Nucleus.
Calculated MW:
61kDa
Observed MW:
60kDa
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.
Purification Method
Affinity purification
Gene ID
2534
RRID
AB_2771129
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-Fyn-S21 Rabbit pAb (CABP0510) at 1:1000 dilution. A-431 cells were treated with 10% FBS after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
