The Phospho-GSK3alpha-S21 Antibody (CABP0582) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-GSK3alpha-S21 Antibody
SKU:
CABP0582
Size:
100μL, 20μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GSK3 alpha, GSK3A, Phospho-GSK3α-S21
Positive Sample:
293 treated with Insulin, HeLa treated with Insulin
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.
Purification Method
Affinity purification
Gene ID
2931
RRID
AB_2771150
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293 and HeLa cells, using Phospho-GSK3α-S21 Rabbit pAb (CABP0582) at 1:1000 dilution. 293 cells were treated with Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated with Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
Western blot analysis of lysates from 293 cells, using Phospho-GSK3α-S21 Rabbit pAb (CABP0582) at 1:1000 dilution or GSK3α antibody (A0645). 293 cells were treated with Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
