The Phospho-GYS1-S641 Antibody (CABP0877) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the phosphorylated form of GYS1, this antibody enables the detection and analysis of GYS1 activation in various cell types, making it an excellent choice for studies in metabolism and cellular signaling pathways.GYS1 is a key enzyme involved in glycogen synthesis and glucose metabolism, with phosphorylation at serine 641 playing a role in regulating its activity. Dysregulation of GYS1 phosphorylation has been linked to metabolic disorders, such as diabetes and obesity, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-GYS1-S641 Antibody
SKU:
CABP0877
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PASV P
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GSY, GYS, Phospho-GYS1-S641
Positive Sample:
293T, NIH/3T3, C6
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
84kDa
Observed MW:
100kDa
The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2997
RRID
AB_2771156
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-GYS1-S641 Rabbit pAb (CABP0877) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from 293T cells, using Phospho-GYS1-S641 pAb (CABP0877) at 1:1000 dilution or Glycogen synthase 1 (GYS1) antibody (CAB2519). 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
