Description
H3F3B Antibody (CAB10220)
The H3F3B Antibody (CAB10220) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically targeting the Histone H3.3 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for studies in epigenetics, cancer research, and developmental biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | H3F3B Antibody |
| SKU: | CAB10220 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | MART KQTA RKST GGKA PRKQ LATK AARK SAPS TGGV KKPH RYRP GTVA LREI RRYQ KSTE LLIR KLPF QRLV REIA QDFK TDLR FQSA AIGA LQEA SEAY LVGL FEDT NLCA IHAK RVTI MPKD IQLA RRIR GERA | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | H3-3A, H3.3B, H3F3B, BRYLIB2 |
| Positive Sample: | A-549, SW620, U-87MG, MCF7, LO2 |
| Cellular Localization: | Chromosome, Nucleus. |
| Calculated MW: | 15kDa |
| Observed MW: | 15kDa |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17.
| Purification Method | Affinity purification |
| Gene ID | 3021 |
| RRID | AB_2757744 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using H3F3B Rabbit pAb (CAB10220) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
