The Phospho-Histone H3-T6 Antibody (CABP0899) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbits as the host organism, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the phosphorylated form of Histone H3 at threonine 6, this antibody enables precise detection and analysis of this critical histone modification in various cell types.The phosphorylation of Histone H3 at threonine 6 is known to play a role in regulating chromatin dynamics and gene transcription, making it a key player in cellular processes such as cell division, DNA repair, and apoptosis.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Phospho-Histone H3-T6 Antibody
SKU:
CABP0899
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
KQTA R
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2863818
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C2C12 cells, using Phospho-Histone H3-T6 Rabbit pAb (CABP0899) at 1:400 dilution. C2C12 cells were treated with nocodazole (50 ng/ml) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-Histone H3-T6 Rabbit pAb (CABP0899) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using Phospho-Histone H3-T6 Rabbit pAb (CABP0899) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using Phospho-Histone H3-T6 Rabbit pAb (CABP0899) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-T6 Rabbit pAb (CABP0899) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-T6 Rabbit pAb (CABP0899) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
